The use of medicinal plants is as old as human life. Old documents of medicine and Pharmacy are include experiences and valuable information of treatment plant. Until the last few decades what was used as medicine came mainly from plant and natural resources. Iranian traditional medicine Mainly based on the use of medicinal plants. Medicinal plants are rooted in our tradition, Development of traditional medicine in the country is not only a strategy for development of medicinal plants industry, But also according to the recommendations of the World Health Organization as one of the suitable methods for the public easy access to medicine is safe and cheap prices. One of the ways you can get valuable information regarding medicinal plants is review of the regional flora and evaluation of Ethnobotany. In this study, we have collected and identified the plants in Gardaneh Rokh in Charmahal and Bakhtiari Province. To this end, during 2012 to 2014,the plants of this region where collected and systematically identified. furthermore traditional applications of the collected plants were recorded through interviewing the native people of the region. the result indicated that most plants of the region are valuable medicinal plants. Therefore, it seems that plants of the region deserves further studies.