Background and Purpose: Henna is used as a medicine in traditional medicines of some countries to treat burns, wounds, some fungal infections, and inflammations. Considering the importance of wound healing and the fact that not treating open wounds may have irreparable consequences; various studies have been conducted on wound healing and as a result, various materials have been prepared and introduced as wound ointments, most of which have been in the form of herbal and sometimes chemical formulations, but so far none have been able to be recommended as an effective medicine. For this reason, this project attempts to investigate the wound healing properties of henna in combination with several other plants.
Materials and Methods: Arnebia euchroma, Curcuma zadoaria, Ferula gummosa, Acacia arabica, Lawsonia inermis, Azadirachta indica, wax, sesame oil, mice and anesthetics were bought. Extraction and purification of the extracts of the mentioned natural plants were carried out. The resulting mixture was placed on a heater-stirrer with sesame oil and wax to dissolve them. The effect of the ointment on burned skin in mice was investigated. Lipid peroxidation tests, antioxidant potency, tissue evaluation and stability tests were also performed.
Results: Second-degree burns were induced in mice and treated with the prepared ointment. The wound area in the control group reached 16.29 mm2, while in the treatment group this area reached 4.201 mm2. Moreover, lipid peroxidation showed a significant decrease compared to the control group, while antioxidant potency increased. In the microscopic evaluation of the tissue, more angiogenesis and less inflammation were observed compared to the control group. In the stability test of the ointment; uniformity, pale yellow color, smell of Barijeh essential oil, weight of 50, PH=3, viscosity of 23000 and dry matter of 80 were observed.
Conclusion: The ointment prepared with Henna extract and the mentioned plants had a very good healing effect compared to the positive and negative control groups. |
